|
KMID : 0900920020260030263
|
|
Korean journal of Animal Reproduction
2002 Volume.26 No. 3 p.263 ~ p.273
|
|
|
Effects of beta-Mercaptoethanol on lipid Peroxidation and Fertilization Ability In Vitro by Xanthine-Xanthine Oxidase System in Pig
|
|
Cheong Hee-Tae
Lee Chang-Hee
Ryu Il-Sun
Yang Boo-Keun
Kim Choung-Ik
Park Choon-Keun
|
|
|
Abstract
|
|
|
|
This study was undertaken to evaluate the effects of beta-mercaptoethanol (beta-ME) on lipid peroxidation and fertilization ability in vitro by xanthine (X) - xanthine oxidase (XO) system in boar spermatozoa frozen-thawed. The boar spermatozoa were treated with X and/or XO, and the spermatozoa viability were measured by the eosin-nigrosin stain method. In control group, level of vitality in boar spermatozoa were higher than in medium with X, XO and X+XO groups. No significant differences, however, were observed under the all conditions. The percentage of spermatozoa that reached acrosome reaction were significantly (P<0.05) higher in sperm treated without that than with beta-ME under the all conditions. On the other hand, when spermatozoa were inseminated in medium with X and/or XO, the penetration rates in all conditions were higher in medium with that than without beta-ME. However, significant differences were not observed between medium with and without beta-ME. The lipid peroxidation of sperm was evaluated on the basis of malondialdehyde (MDA) production. The MDA were higher in sperm treated without that than with beta-ME under the above all conditions. However, significant differences were not observed between medium with and without beta-ME. Sperm-SH group were higher detected in medium with that than without beta-ME under the all conditions. The activity of sperm binding to Bona pellucida was also evaluated through binding to salt-stored porcine oocytes. In control group, sperm binding to zona pellucida were significantly (P<0.05) higher than in medium with X+XO groups. The sperm binding in all conditions were higher in medium with that than without beta-ME. However, significant differences were not observed between medium with and without beta-ME. These results suggest that addition of beta-ME in X-XO system may play a positive role in improving of fertilization ability in vitro.
|
|
|
KEYWORD
|
|
|
beta-Mercaptoethanol, Lipid peroxidation, Fertilization ability, Xanthine-xanthine oxidase system, Pig
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|